Simultaneous measurements of atmospheric water vapor were made by the Millimeter-wave Imaging Radiometer (MIR), Raman lidar, and rawinsondes. Two types of rawinsonde sensor packages (AIR and Vaisala) were carried by the same balloon. The measured water vapor profiles from Raman lidar, and the Vaisala and AIR sondes were used in the radiative transfer calculations. The calculated brightness temperatures were compared with those measured from the MIR at all six frequencies (89, 150, 183.3 ± 1, 183.3 ±3, 183.3 ±7, and 220 GHz). The results show that the MIR-measured brightness temperatures agree well (within ±K) with those calculated from the Raman lidar and Vaisala measurements. The brightness temperatures calculated from the AIR sondes differ from the MIR measurements by as much as 10 K, which can be attributed to low sensitivity of the AIR sondes at relative humidity less than 20%. Both calculated and the MIR-measured brightness temperatures were also used to retrieve water vapor profiles. These retrieved profiles were compared with those measured by the Raman lidar and rawinsondes. The results of these comparisons suggest that the MIR can measure the brightness of a target to an accuracy of at most ±K and is capable of retrieving useful water vapor profiles.