1. Introduction
The upper-ocean mixed layer (ML) is generally characterized by a vertically homogeneous profile of temperature, salinity, and density. This layer results from vertical mixing near the surface is promoted by various processes—wind stirring, waves, and turbulence—generated by vertical shear or nighttime convective mixing. Hence, the ML is important in establishing the mean state and variability of the World Ocean because it acts as a buffer between the atmosphere and the interior ocean for exchanging heat, momentum, freshwater, and gases (Kantha and Clayson 1999; Wunsch and Ferrari 2004; Vallis 2006). Because of the increasing attention upon climate change or global warming, the ML has been extensively studied by both oceanographers and meteorologists (e.g., Deser et al. 1996; Hanawa and Talley 2001; Yu and Weller 2007; Oka et al. 2015). The ML can be basically characterized by its thickness, typically called mixed layer depth (MLD). The MLD influences the evolution of sea surface temperature (e.g., Carton et al. 2008) and modulates the heat content of the ML (e.g., Godfrey and Lindstrom 1989; Dong et al. 2008), which is highly related to a wide variety of phenomena, from tropical cyclone formation and phytoplankton bloom critical depth theory to climate variability. Therefore, accurate determination of the MLD is a priority in studying these important physical and biology processes in the upper ocean.
A significant amount of work has been undertaken in developing methods of reliable calculation of the MLD (Defant 1936; Wyrtki 1964; Levitus 1982; Lukas and Lindstrom 1991; Brainerd and Gregg 1995; Obata et al. 1996; Kara et al. 2000; Lavender et al. 2002; Thomson and Fine 2003; Lorbacher et al. 2006; Holte and Talley 2009; Chu and Fan 2011). Temperature, salinity, and density are approximately homogeneous within the ML, and they present sharp gradients beneath the ML. The aforementioned studies are aimed at automatically specifying the lower bound of the ML (i.e., its intersection with the underlying water column) by implementing some criteria on discretized temperature or density profiles. Among these methods, the ones based on a threshold value from a reference depth of 10 m have been most widespread because they are simple and can presumably be applied to profiles with various depth resolutions (Ohno et al. 2004). These threshold methods are based on the changes in temperature and density [see Kara et al. (2000), Thomson and Fine (2003), de Boyer Montégut et al. (2004), Lorbacher et al. (2006), and Holte and Talley (2009) for summaries of various thresholds] or in their gradients (Lukas and Lindstrom 1991). These threshold methods have been largely employed to evaluate the ML of single profiles and to generate the climatological field (Kara et al. 2003; de Boyer Montégut et al. 2004; Holte and Talley 2009). Generally speaking, the depth at which the local density (temperature) starts differing from the surface density (temperature) by 0.01–0.03 kg m−3 (0.01°–1°C) is considered as an MLD proxy. There are also many other relatively sophisticated definitions, such as using a hybrid method based on the results from the threshold methods (Holte and Talley 2009), a step-function least squares regression method and integral depth-scale method used for coarse-resolution profiles (Freeland et al. 1997; Thomson and Fine 2003), a split–merge method based on profile shape (Pavlidis and Horowitz 1974; Thomson and Fine 2003) or dissolved oxygen profiles (Castro-Morales and Kaiser 2012), a curvature-based method (Lorbacher et al. 2006), and a linear optimal fitting method (further developed as a maximum angle method) (Chu and Fan 2010, 2011).
Depending critically upon the choice of threshold value, the threshold methods are somewhat subjective as a result of the selection of fixed threshold values. As was tested by Brainerd and Gregg (1995), Kara et al. (2000, 2003), de Boyer Montégut et al. (2004), Lorbacher et al. (2006), Holte and Talley (2009), and Ohno et al. (2009) for profiles of temperature or density, a threshold chosen subjectively for one region or one season might not be applicable to another region or season. For example, Ohno et al. (2009) found that the optimal threshold varies from 0.06 kg m−3 in the tropics and subtropics to 0.09 kg m−3 in the eastern subarctic. Moreover, as was pointed out by de Boyer Montégut et al. (2004) and Lorbacher et al. (2006), the shortcomings of the threshold methods are obvious, especially regarding profiles with gradual pycnoclines. Typically, a smaller threshold can lead to a shallower MLD, and the threshold methods often miss the MLD when a small jump occurs in the ML. Therefore, it is difficult to determine a universal threshold value for all ocean profiles.
In order to objectively determine the MLD, a few—and less dependent on fixed criteria— complicated methods have been developed. The split–merge method proposed by Thomson and Fine (2003) is based on the optimal analysis of linear segments of a density profile. They found that their method performed similarly to the threshold methods, but with a slight improvement in depth determination. The curvature method, as tested by Chu and Fan (2011), is less valid when identifying the MLD from the glider data. As the curvature method deals with the second derivative, it was reported that this method often produced the problematic MLD with the “wriggly” profiles (Lorbacher et al. 2006; Chu and Fan 2011). The maximum angle method seemed to determine the MLD accurately when applied to Seaglider data; however, it was suggested to be unsuitable for low-resolution profiles because of its dependence on linear regression (Chu and Fan 2011). As the shapes of the mixed layer and the underlying water are often complex as a result of the variety of dynamic processes, how to reliably determine the MLD becomes much more important.
In this study we proposed a new objective method, referred to as the “relative variance method” (Rel Var), that is based on the automated estimation, over the depth span from the surface to each profile depth, of the standard deviation normalized by the maximum variation of the temperature or density. The depth of the minimum value of the relative variance is identified as the MLD. The effectiveness of this new method is evaluated using the World Ocean Circulation Experiment (WOCE) data against the results of other available methods. The performance of the new method is indicated to be the optimal one and is less affected by data noise and depth resolution. The outline of this paper is as follows. In section 2 we describe the hydrographic data and the methodology in details. In section 3 we test the influence of the random noise on the new method and examine its performance in comparison with other available methods using some examples and the profiles of different depth resolutions of the WOCE section A05. In section 4 we present the global MLD variability determined by the threshold and objective methods. A summary is presented with a discussion in section 5.
2. Data and method
a. Hydrographical data
We used temperature and salinity data from the WOCE, the U.S. Climate Variability and Predictability Program (CLIVAR), and other programs, which were downloaded from the CLIVAR and Carbon Hydrographic Data Office website (https://cchdo.ucsd.edu/) on 9 July 2015. These data include 723 sections and 34 705 stations between 2 April 1980 and 17 July 2014. The data quality is controlled by the following criteria:
- There should exist at least two data points between 10 and 40 dbar.
- The maximum measured depth is ≥50 dbar.
- The mean depth resolution is ≤2 dbar; 673 profiles cannot meet this requirement and are ruled out. The remaining profiles have depth resolutions of 1 or 2 dbar, in particular 78.3% of them have a depth resolution of 2 dbar. As discussed below, the relative variance method is less affected by the depth resolution; however, this criterion ensures that the depth resolutions of all profiles are consistent.
- The shallowest measurement depth of a profile is ≤20 dbar.
- The maximum temperature (salinity, density) difference in the depth range between the sea surface and 20 dbar should be less than the maximum respective difference in the depth range from 20 dbar to the deepest depth of the examined profile
- The temperature difference of the whole profile is ≥ 1°C.
These criteria leave a final dataset of 32 686 CTD profiles by removing some profiles that are useless, erroneous, or contain atypical data for the MLD computation. The coverage of these profiles is shown in Fig. 1.

Spatial distribution of WOCE data used in the study. Data are generally denser over the Atlantic Ocean. Section A05 is marked (red).
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
To assess the impact of depth resolution on the determination of the MLD, we applied different methods to the transatlantic hydrography section at 24.5°N in 2010 (WOCE section A05, ExpoCodes: 74DI20100106), where only one CTD cast was considered at each station and a total of 135 profiles were obtained. The temperature was recorded by Sea-Bird 911plus CTD with a sampling rate of 25 Hz. Some raw data had spurious temperature values occurring slightly beneath the sea surface (
b. Methodology











































Determination of MLD using the relative variance method on (a) the potential temperature profile at station 65 (24.5°N, 56.7°W) on 23 Jan 2010 along section A05. The depth of minimum
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
As the ML has a limited thickness, it is much more realistic to seek the ML base at the depth
3. The performance of the new method
a. Influence of noise

As shown in Fig. 3a, this artificial temperature profile is uniform for the upper 72 dbar and is exponentially decaying with increasing depth, corresponding to an MLD of 72 dbar. Next, the temperature profile is added with the normally distributed random noise of amplitude

(a) Artificial temperature profiles
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
In addition, we add the normally distributed random noise to the temperature profile shown in Fig. 2a. Similar to the abovementioned artificial profile, as shown in Fig. 3d, the MLD determined by the proposed method keeps constant (89 dbar) at a low noise level (
b. Comparison with other methods
To further evaluate the performance of the relative variance method, a comparison of this method with the six other currently used methods is being done. These methods are described as follows.
- Difference method (
): Since this method is simple and stable in determining MLD, it has been extensively applied. As recommended by de Boyer Montégut et al. (2004), the temperature difference of 0.2°C and the density difference of 0.03 kg m−3 (to the reference depth of 10 dbar) are the optimal thresholds when taking the global MLD distribution into consideration. Here, the reference depth is alternatively selected as the sea surface for consistency with the other methods. - Difference-interpolation method (Dif Int): A linear interpolation between observed levels is additionally employed to the result of the difference method to determine the MLD more accurately (de Boyer Montégut et al. 2004).
- Gradient method (
): Following the suggestion by Dong et al. (2008), a temperature gradient criterion of 0.025°C dbar−1 and a potential density gradient criterion of 0.0005 kg m−3 dbar has been used to determine the MLD. - Hybrid method (
): From a suite of possible MLD values assembled by the difference and gradient methods, a complicated fitting algorithm is employed to approximate the MLD (Holte and Talley 2009). The detailed algorithm of the method are publicly available. - Curvature method (
): In this method one examines the second derivative of temperature (density) with respect to depth, referred to in the literature as “curvature” (Lorbacher et al. 2006). For each data point, a curvature value is being estimated and the first (as one scans the profile downward from the sea surface) local maximum of curvature (within some tolerance of one standard deviation of temperature/density) is being defined as the lower MLD base. The algorithm of the method is publicly available. - Maximum angle method (Max Ang): According to this method, two linear regressions are applied to the temperature/density profile, one on the data points above and one beneath each data depth. The depth, at which the angle formed by the two aforementioned linear fits is maximum, is identified as the MLD (Chu and Fan 2011).
1) Example profiles
Here, we first show the efficiency of these methods in determining the MLD of four temperature profiles from the section A05. As shown in Fig. 4a (station 65), when the ML and the underneath water column are well separated, all the methods can approximately determine the MLD. The MLD is visually inspected to be 89 dbar. It is estimated to be within the range between 87.9 dbar by the curvature method and 94.0 dbar by the difference method. When the dynamics are complex in the upper ocean, leading to highly stratified water columns occurring in several depth ranges (Fig. 4b, station 69), the determined MLDs by different methods are much scattered. The base of a homogeneous layer is identified by the hybrid, curvature, and relative variance methods at 61, 65, and 59 dbar, respectively. The difference and difference-interpolation methods also approximately capture the MLD, which are 72.0 and 70.5 dbar, respectively. This is consistent with the findings in previous studies (Lorbacher et al. 2006; Holte and Talley 2009); that is, the estimations from the difference and difference-interpolation methods are generally deeper than the actual MLD. The determinations by the maximum angle and gradient methods are 152 and 156 dbar, respectively, which correspond to the top depth of deeper stratified water column. In the case where the temperature changes gradually with increasing depth—for example, in station 13 (Fig. 4c)—the hybrid method identifies the MLD as 13 dbar, which seems too shallow with visual inspection. The curvature method finds the MLD to be 26 dbar. The relative variance method finds the almost homogeneous temperature depth range with the determined MLD of 71 dbar. The MLD determined by the difference and difference-interpolation methods is decided by the threshold values, which are 76.0 and 75.2 dbar, respectively. The maximum-angle and gradient methods capture the top of the deeper stratified water column; their MLDs are estimated to be 126 and 124 dbar, respectively. When the temperature signal gets more complicated in the ML—for example, the temperature inversions occurred at station 60 (Fig. 4d)—the curvature and maximum angle methods are affected by the temperature inversion around

Potential temperature profiles (circles with black lines) and the corresponding QI (gray lines) defined in Eq. (7b) with the vertical space
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1






In the example profile shown in Fig. 4a, it is found that QI corresponds to the maximum value when approaching the base of a highly homogeneous ML. The maximum QI (
2) Influence of depth resolution














(a) The mean MLD
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
In particular, the function
Statistics of H and QI for all stations along the section A05, showing the corresponding mean

Additionally, the dependences of QI, its mean

(a) The mean quality index
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
4. Application to the global ocean
The new method is applied to the WOCE data shown in Fig. 1 to exhibit the global MLD distribution. Both temperature and density profiles are considered to determine MLD values, referred to as
a. Probability density functions of global MLD
The probability density functions (PDFs) of the MLD from

PDFs of MLD (a)
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
Global statistics (mean

Figure 8 shows the PDFs of the MLD difference,

(a) PDFs of MLD difference,
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1

Example profiles of (a) potential temperature and (b) potential density from the equatorial Atlantic Ocean (4.3°S, 34.8°W) on 21 Oct 1990. Difference-interpolation (cyan), hybrid (blue), curvature (red), and relative variance (black) methods produce MLDs of (a) 94, 94.15, 68.5, and 68 dbar, and (b) 72, 70.7, 67.8, and 70 dbar.
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
Figure 10 shows the PDFs of

PDFs of QI from (a) temperature and (b) density profiles with the difference-interpolation (cyan), hybrid (blue), curvature (red), and relative variance (black) methods.
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
b. Global MLD variability
To gain further insight into the effectiveness of the relative variance method (as compared to the effectiveness of the other methods) in accurately determining MLDs, it is worthwhile to additionally examine both the temporal evolution and the spatial distribution of the various MLD estimates. As indicated in Fig. 1, the WOCE data are relatively sparse in the global ocean; hence, a zonal mean is taken. Since the MLDs are characterized by strong seasonal variation, they are further averaged into monthly bins.
Figure 11 shows the spatial and temporal variation of the MLDs estimated by each of the four aforementioned methods. The blank regions indicate the impossibility of reasonable interpolation owing to data sparsity. In particular, we can see that the month–latitude patterns of MLD are similar for the four methods. In these patterns the seasonality of the MLD is well exhibited, and the seasonal variations of the MLD are almost opposite in the northern and southern hemispheres (as expected). The MLD maxima are found in the wintertime between 45° and 60°S, including the Southern Ocean, and between 30° and 60°N corresponding to the North Atlantic Deep Water formation region. The MLD minima are found in the summertime midlatitude regions. The seasonal cycle of MLD in the region of the Antarctic Circumpolar Current is especially pronounced with several hundreds of decibars in winter and dozens of decibars in summer. Moreover, the pattern of temperature-based MLD,

Monthly variations of zonally integrated MLD, H, from temperature and density profiles with different methods. (a), (c), (e), (g)
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
Figure 12 shows the spatial and temporal variation of QI from different methods. Lorbacher et al. (2006) suggested that QI has large values in the hemispheric summer and fall when a sharp gradient at the base of the ML is present. This feature can be observed in the

Monthly variations of zonally integrated QI from temperature and density profiles with different methods. (a), (c), (e), (g)
Citation: Journal of Atmospheric and Oceanic Technology 35, 3; 10.1175/JTECH-D-17-0104.1
5. Summary and discussion
In this study a new objective method—the relative variance method—is presented for assessing MLDs on the basis of individual temperature or density profiles. According to this method, in particular, the relative variance is the ratio where the numerator is the standard deviation value of the property (temperature, salinity) over the depth regime ranging from the surface to the “current” depth, and the denominator is the difference between the maximum and the minimum property values over the abovementioned depth regime; the MLD is defined as the depth at which the relative variance is at its minimum. For evaluating the effectiveness of the new method, the influence of random noise is examined and a comparison of this method with other available methods is conducted, including the threshold methods [difference, difference-interpolation (de Boyer Montégut et al. 2004), gradient (Dong et al. 2008), and hybrid (Holte and Talley 2009) methods] and the objective methods [curvature (Lorbacher et al. 2006) and maximum angle (Chu and Fan 2011) methods]. All methods are applied on an affluence of WOCE data.
When the example temperature profile is added with random noise (noise level ≤ 5%), the MLD determined by the new method is changed less than 2 dbar even the noise amplitude is as large as 0.32°C (Fig. 3d). The profiles are often contaminated by instrument noises, measurement errors, and ship heave motions, which often lead to spurious inversions or spikes (e.g., Johnson and Garrett 2004; Gargett and Garner 2008); results of the random noise testing suggest that the new method is less affected by data fluctuations and therefore identifies a more reliable MLD. Regarding the temperature data of the WOCE section A05, where the depth resolution of the profiles is resampled from 0.04 to 25 dbar, the MLDs determined by the relative variance and the difference-interpolation method are much more consistent with the visually inspected MLD value than the MLDs by other methods. However, the relative variance method derives the most stable MLD and the largest QI among all the methods. These results confirm that the relative variance method is the optimal one for capturing the intersection of a homogeneous ML with an underlying water of sharp gradient, with profiles of any depth resolution.
When applied to the WOCE data of the global ocean, all the methods were found to yield nearly coincident PDFs of
Finally, the leading effectiveness of the relative variance method is further confirmed by the fact that the MLD of 94% of the WOCE temperature profiles can be determined with this method for
We thank Dr. Brian King for offering the raw data from section A05. We specially thank the anonymous reviewers for their helpful suggestions. This work was supported by the China NSF (41476167, 41406035, and 91752108); the NSF of Guangdong Province, China (2016A030311042); and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA11030302).
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